Characterizing Avian Escherichia coli Isolates with Multiplex Polymerase Chain Reaction
نویسندگان
چکیده
منابع مشابه
Characterizing avian Escherichia coli isolates with multiplex polymerase chain reaction.
Colibacillosis caused by Escherichia coli infections account for significant morbidity and mortality in the poultry industry. Yet, despite the importance of colibacillosis, much about the virulence mechanisms employed by avian E. coli remains unknown. In recent years several genes have been linked to avian E. coli virulence, many of which reside on a large transmissible plasmid. In the present ...
متن کاملMolecular typing of avian Escherichia coli isolates by enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR)
BACKGROUND: Colibacillosis is one of the most economically important diseases of poultry worldwide. OBJECTIVES: This study was conducted to examine the clonal relatedness and typing of 95 avian Escherichia coli isolates by ERIC-PCR. METHODS: Sixty-three E. coli isolates from two common manifestations of colibacillosis (yolk sac infection and colisepticemia) and 32 isolates from feces of apparen...
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AIM This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance. MATERIALS AND METHODS A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR). RESULTS All fecal samples were positive for E. coli. All the E....
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We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies...
متن کاملmolecular typing of avian escherichia coli isolates by enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (eric-pcr)
background: colibacillosis is one of the most economically important diseases of poultry worldwide. objectives: this study was conducted to examine the clonal relatedness and typing of 95 avian escherichia coli isolates by eric-pcr. methods: sixty-three e. coli isolates from two common manifestations of colibacillosis (yolk sac infection and colisepticemia) and 32 isolates from feces of apparen...
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ژورنال
عنوان ژورنال: Avian Diseases
سال: 2003
ISSN: 0005-2086,1938-4351
DOI: 10.1637/7030